Rovers are expected to make several changes to the team that saw off Morcambe in front off home supporters on Saturday afternoon, with Emmanuel Monthe and Jack Fitzwater likely to start.
Youngsters Jordan Simpson and Jordan Morris have also been named in contention to feature, with both having already played as substitutes earlier in the competition.
The Green Devils have already qualified for the next round and currently sit top of the group on goal difference, having taken maximum points from their opening two fixtures against Newport County and local rivals Cheltenham Town.
An encouraging run of recent form has seen the Gloucestershire outfit win four of their last six in all competitions, following a stretch of seven games without triumph. Rovers also have the chance to win successive fixtures at home for only the second time this season.
The visitors are likely to prove a tough test and can also boast a 100% record in the tournament, having won both their games on the road by a 2-1 score line.
Dutch midfielder Kees De Boer has been singled out as the main threat from the opposition, who are also likely to field Arnór Guðjohnsen, younger brother of Icelandic legend Eidur.